Figure 1

Expression of phosphorylated RTKs in RMS cell lines. Multiple RTKs are detected in RMS cell lines RD (A), RW9019 (B), RH30 (C) and normal muscle cell line HASMC (D). Whole-cell extracts were incubated on RTK antibody arrays and phosphorylation status was determined by subsequent incubation with anti-phosphotyrosine horseradish peroxidase. Each RTK is spotted in duplicate and the pairs of dots in each corner are positive controls. Each pair of positive RTK dots is denoted by a red numeral, with the identity of the corresponding RTKs listed below the arrays. E, thirteen overexpressed RTKs were semi-quantified with Image J software (NIH, USA).