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Table 1 Proliferation yield and phenotype analysis of lymphocytes expanded from metastatic lymph nodes of eight patients with melanoma

From: Value of large scale expansion of tumor infiltrating lymphocytes in a compartmentalised gas-permeable bag: interests for adoptive immunotherapy

Patients

Initial cell number

Final cell number

Fold expansion

CD4a

CD8a

CD8+/IFNγ+ TIL

 

(×106)

(×106)

 

%

Nb (106)

%

Nb (106)

%

Nb (106)

01 (plates)

0.39

794 (±501)

2035 (±1287)

56 (±8)

439 (±257)

43 (±9)

342 (±257)

2 (±1)

5 (±2)

01 (bag)

0.39

1128 (±445)

2892 (±1141)

11 (±2)

123 (±35)

85 (±2)

953 (±366)

14 (±1)

129 (±45)

02 (plates)

0.39

982 (±50)

2518 (±128)

80

797 (±49)

19 (±4)

191 (±54)

2 (±1)

3 (±1)

02 (bag)

0.39

1215 (±153)

3115 (±393)

17 (±6)

207 (±50)

80 (±3)

981 (±166)

5 (±1)

45 (±14)

03 (plates)

0.39

1698 (±182)

4352 (±466)

5 (±1)

85 (±19)

95 (±1)

1612 (±172)

15 (±8)

241 (±106)

03 (bag)

0.39

773 (±366)

1984 (±938)

1 (±0)

6 (±4)

99 (±0)

766 (±362)

15 (±5)

107 (±31)

04 (plates)

0.39

895 (±217)

2296 (±557)

76 (±2)

676 (±158)

21 (±2)

192 (±55)

1 (±1)

2 (±0)

04 (bag)

0.39

600 (±240)

1539 (±615)

61 (±18)

387 (±257)

33 (±16)

180 (±94)

2 (±1)

3 (±2)

05 (plates)

0.39

989 (±180)

2537 (±463)

24 (±4)

236 (±66)

75 (±4)

741 (±132)

6 (±1)

46 (±9)

05 (bag)

0.39

969 (±88)

2484 (±225)

8 (±3)

76 (±17)

90 (±2)

870 (±100)

12 (±4)

107 (±44)

06 (plates)

0.39

910 (±199)

2334 (±509)

42 (±4)

387 (±119)

60 (±7)

533 (±60)

5 (±1)

26 (±3)

06 (bag)

0.39

1323 (±152)

3393 (±389)

10 (±5)

132 (±71)

91 (±5)

1205 (±162)

23 (±2)

281 (±58)

07 (plates)

0.39

1126 (±199)

2889 (±509)

24 (±5)

274 (±73)

75 (±2)

851 (±174)

16 (±4)

132 (±27)

07 (bag) (plates))

0.39

795 (±174)

2039 (±446)

6 (±1)

46 (±18)

93 (±1)

737 (±162)

26 (±8)

184 (±29)

08 (plates)

0.39

772 (±123)

1982 (±316)

71 (±12)

552 (±142)

31 (±13)

234 (±81)

2 (±1)

4 (±1)

08 (bag)

0.39

571 (±163)

1465 (±417)

46 (±17)

281 (±182)

54 (±14)

296 (±24)

5 (±3)

14 (±9)

  1. For each patients, 3 or 4 independent cell expansions were conducted in parallel in bags and in plates and analysed at day 20. Values are the means obtained from these independent cell amplifications. Bracketed values show the standard deviation.
  2. a, gated on lived CD3 + cells.