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Table 1 Proliferation yield and phenotype analysis of lymphocytes expanded from metastatic lymph nodes of eight patients with melanoma

From: Value of large scale expansion of tumor infiltrating lymphocytes in a compartmentalised gas-permeable bag: interests for adoptive immunotherapy

Patients Initial cell number Final cell number Fold expansion CD4a CD8a CD8+/IFNγ+ TIL
  (×106) (×106)   % Nb (106) % Nb (106) % Nb (106)
01 (plates) 0.39 794 (±501) 2035 (±1287) 56 (±8) 439 (±257) 43 (±9) 342 (±257) 2 (±1) 5 (±2)
01 (bag) 0.39 1128 (±445) 2892 (±1141) 11 (±2) 123 (±35) 85 (±2) 953 (±366) 14 (±1) 129 (±45)
02 (plates) 0.39 982 (±50) 2518 (±128) 80 797 (±49) 19 (±4) 191 (±54) 2 (±1) 3 (±1)
02 (bag) 0.39 1215 (±153) 3115 (±393) 17 (±6) 207 (±50) 80 (±3) 981 (±166) 5 (±1) 45 (±14)
03 (plates) 0.39 1698 (±182) 4352 (±466) 5 (±1) 85 (±19) 95 (±1) 1612 (±172) 15 (±8) 241 (±106)
03 (bag) 0.39 773 (±366) 1984 (±938) 1 (±0) 6 (±4) 99 (±0) 766 (±362) 15 (±5) 107 (±31)
04 (plates) 0.39 895 (±217) 2296 (±557) 76 (±2) 676 (±158) 21 (±2) 192 (±55) 1 (±1) 2 (±0)
04 (bag) 0.39 600 (±240) 1539 (±615) 61 (±18) 387 (±257) 33 (±16) 180 (±94) 2 (±1) 3 (±2)
05 (plates) 0.39 989 (±180) 2537 (±463) 24 (±4) 236 (±66) 75 (±4) 741 (±132) 6 (±1) 46 (±9)
05 (bag) 0.39 969 (±88) 2484 (±225) 8 (±3) 76 (±17) 90 (±2) 870 (±100) 12 (±4) 107 (±44)
06 (plates) 0.39 910 (±199) 2334 (±509) 42 (±4) 387 (±119) 60 (±7) 533 (±60) 5 (±1) 26 (±3)
06 (bag) 0.39 1323 (±152) 3393 (±389) 10 (±5) 132 (±71) 91 (±5) 1205 (±162) 23 (±2) 281 (±58)
07 (plates) 0.39 1126 (±199) 2889 (±509) 24 (±5) 274 (±73) 75 (±2) 851 (±174) 16 (±4) 132 (±27)
07 (bag) (plates)) 0.39 795 (±174) 2039 (±446) 6 (±1) 46 (±18) 93 (±1) 737 (±162) 26 (±8) 184 (±29)
08 (plates) 0.39 772 (±123) 1982 (±316) 71 (±12) 552 (±142) 31 (±13) 234 (±81) 2 (±1) 4 (±1)
08 (bag) 0.39 571 (±163) 1465 (±417) 46 (±17) 281 (±182) 54 (±14) 296 (±24) 5 (±3) 14 (±9)
  1. For each patients, 3 or 4 independent cell expansions were conducted in parallel in bags and in plates and analysed at day 20. Values are the means obtained from these independent cell amplifications. Bracketed values show the standard deviation.
  2. a, gated on lived CD3 + cells.