Figure 5

Levels of tumor necrosis factor-alpha (TNF-α) in the supernatant of cell culture and activities of Cathepsin G of polymorphonuclear neutrophils (PMN). Cells were treated and challenged with vehicle (A), treated with dexamethasone (Dx) and challenged with lipopolysaccharide (LPS) (B), treated with pyrrolidine dithiocarbamate (PDTC) and challenged with LPS (C), or treated with vehicle and challenged with LPS (D). The levels of TNF-a were measured 0, 1, 2, 4, 6, 9 and 12 hours after treatment and challenge, while activities of Cathepsin G in PMNs were measured 12 hours after treatment and challenge.