Figure 3
Effect of RVL on IL-18 secretion from tumor-activated HSE cells in vivo and in vitro. A) IL-18 levels were determined in serum samples obtained from suprahepatic vein blood on the 18th hour after intrasplenic injection of 3 × 105 B16M cells. Some mice received 1 mg/kg/day RVL via intragastic tube 5 days prior to cancer cells injection. B) Primary cultured HSE cells were incubated in the presence or absence of 2.5 μM RVL or recombinant VEGF antibody for 30 min, and then with either B16M-CM or 10 ng/ml recombinant murine VEGF. Eight hours later, the supernatants from treated HSE cells were collected and IL-18 concentration determined by ELISA. Data represent the average values ± SD of 3 independent experiments. *Differences were statistically significant (P < 0.01) to respect to vehicle-treated mice, by Student's two tailed, unpaired t test.