Figure 1
Effect of resveratrol on hepatic metastasis development of intrasplenically-injected B16M. Mice were intrasplenically injected with B16M cells and 1 mg/kg/day RVL was administered via intragastric tube, from day 1 to 12. A and C) Representative picture of livers from vehicle- and RVL-treated mice obtained on the day 12 after cancer cell injection. Metastases are visible as black melanotic nodules. B and D) Representative microscopic pictures of hepatic tissue sections from vehicle- and RVL-treated mouse livers. Tumor-affected hepatic tissue in blue (H) and hepatic metastases in brown due to melanogenic cells (Met). Anti-smooth muscle-alpha antibodies were used to immunohistochemically stain metastasis-associated stromal cells (in red). E) Metastatic volume and number in untreated and RVL-treated mice (n = 10 per group), as percentage of liver occupied by metastases and number of foci per 100 mm3, respectively. Histograms represent average values ± SD of 3 independent experiments. *Differences were statistically significant with respect to vehicle-treated mice (P < 0.01) according to Bonferroni and post-hoc. ANOVA test.