Figure 7

The mRNA and protein expressions of anti-oxidative markers. A) By days 1, 3 and 14 after IR injury, remarkably higher mRNA expression of heme oxygenase (HO)-1 expression in IR and IR + ADMSC groups than in control group, and notably higher expression in IR + ADMSC group than in IR group. 1) Normal vs. day 1, *p < 0.0001 between indicated groups; 2) normal vs. day 3, *p < 0.001 between indicated groups; 3) normal vs. day 14, *p < 0.001 between indicated groups. B) By days 1 and 3 after IR injury, notably higher HO-1 protein expression in control and IR + ADMSC groups than in IR group, and significantly higher expression in IR + ADMSC group than in control group on day 1 after IR injury. Similar expressions, however, noted between control and IR + ADMSC groups by day 3 after IR procedure. By day 14 after IR, substantially higher HO-1 protein expression in IR + ADMSC group than in IR and control groups, and notably higher expression in IR group than in control group. 1) Normal vs. day 1, *p < 0.01 between indicated groups; 2) normal vs. day 3, *p < 0.01 between indicated groups; 3) normal vs. day 14, *p < 0.01 between indicated groups. C) By days 1 and 3 after IR procedure, notably higher NAD(P)H quinone oxidoreductase 1 (NQO1) expression in control and IR + ADMSC groups than in IR group, and significantly higher expression in IR + ADMSC group than in control group; By day 14 after IR injury, notably higher NQO1 protein expression in IR + ADMSC and IR groups than in control group, and expression also significantly higher in IR + ADMSC group than in IR group. 1) Normal vs. day 1, *p < 0.03 between indicated groups; 2) normal vs. day 3, *p < 0.05 between indicated groups; 3) normal vs. day 14, *p < 0.01 between indicated groups. Symbols (*, †, ‡) indicate significance (at 0.05 level) (by Bonferroni multiple comparison post hoc test) (n = 8 for each group).