MARCKS silencing in AoSMCs. (A) No transfection reagent was used. No significant knockdown was achieved using 5 nM or 50 nM MARCKS compared to no reagent control. (B) Transfection reagent HiPerfect combined with 5 nM or 50 nM MARCKS siRNA did not result in significant MARCKS gene knockdown compared to control groups. (C) RNAiMax combined with 50 nM MARCKS siRNA resulted in a significant reduction of MARCKS mRNA levels compared to control groups.