Figure 1

Experimental conditions and total number of samples. Human aortic smooth muscle cells were transfected in the presence or absence of a transfection reagent (HiPerfect™ or RNAiMax™; 0.375 μl/100 μl each). Each group was further divided into four conditions: no siRNA, 50 nM unlabeled control siRNA, and 5 nM and 50 nM of siGLO Red transfection indicator. Each treatment was carried out in quadruplicates and each experiment was repeated three times for a total sample size of 144.