Figure 4

Gene expression analysis. RNA from HuES9E1 and E1-MYC 16.3 MSCs were hybridized to Sentrix HumanRef-8 Expression BeadChip Version 3 and analyzed by Beadstudio and Genespring GX 10. a) Pairwise comparison of gene expression between HuES9.E1 and E1-MYC 16.3 MSCs using Beadstudio analysis. b,c) PANTHER analysis. 161 genes that were over-expressed by > 2-fold and 226 under-expressed genes that were under-expressed by > 2-fold in E1-MYC 16.3 MSCs were analyzed using PANTHER algorithm. The observed frequency of genes for each biological process in each gene set was compared with the reference frequency which, in this case is the frequency of genes for that biological process in the NCBI database. Those biological processes whose observed frequency exceeds the reference frequency with a p < 0.05 are considered significant.