Figure 1

Sorting of human CD8⁺ T lymphocytes and microRNA large scale expression profiling. a) CD8⁺ T cells from healthy volunteers were FACS-sorted based on their expression of CD45RA, CCR7 and CD28 to isolate Naive (CD45RA⁺ CCR7⁺, CD28⁺), E-EM (CD45RA^- CCR7^-, CD28⁺), I-EM (CD45RA^- CCR7^-, CD28^-) and L-EM (CD45RA⁺ CCR7^-, CD28^-). Data shown are gated on CD3⁺ CD8⁺ cells. b) Unsupervised clustering of the expression levels (ΔCt, normalized to RNU44) of 223 unique microRNAs (detected in at least one sample, i.e. Ct < 36) in CD8⁺ T lymphocyte subsets from 3 healthy donors (HD1, HD2, HD3) as measured by TLDA (green: low expression/red: high expression).