Figure 4

Effects of different treatments on tumor infiltration by immune cells, apoptosis and proliferation in LLC tumors. Treated mice were sacrificed 24 hours after last IAA administration. Tumor sections were determined by immunohistochemical staining using specific antibodies against CD4, CD8, Ki-67 and TUNEL assay. The positive cells were scored through light microscopy. After initial scanning under × 100 magnification, positive stained cells in 10 filed under × 400 magnification were counted and the mean number of stained cells was averaged over 10 fields.