Figure 2

Time course of WC007 CRC apoptosis induction by CTL007 in reconstruct. Reconstructs were prepared as in Fig.1. An E: T ratio of 2:1 was used in this assay, reconstructs harvested on day 6 or 8 (3 or 5 days after adding T cells), fixed, processed and enumerated as described in Fig.1. Values represent mean percentage of apoptosis/field (total of 10 fields), of cultures with lymphocytes corrected by the value obtained without lymphocytes, ± SD (bars). Percent apoptotic tumor cells of reconstruct cultures with CTL (▲) were significantly higher than cultures with PHA blast (■) on both days, for cultures with and without separating layer (at p < 0.05 level). Percent apoptotic tumor cells of reconstruct cultures was significantly (p = 0.005) higher on day 5 than on day 3 for cultures with and without separating layer. The separating layer had no significant effect on percent apoptotic cells.