Figure 1
Characteristics of immature dendritic cells that were generated in different culture media. A. Morphology and density of adherent monocytes after 2 h of adhesion in in CellGro or RPMI+5% human AB serum. B. Total number of immature DCs that were generated from 1 × 107 of PBMCs in CellGro or RPMI+5% human AB serum. Each symbol represents an individual experiment, and the horizontal bar indicates the mean value for all experiments. * P value for comparison between tested conditions, P < 0.05. C. Purity (percentage of CD11c positive DCs) and viability (percentage of DAPI negative cells) of DCs generated in CellGro or RPMI+5% human AB serum and activated by tested maturation stimuli.