Table 2 Applied RNAi control experiments - overview
From: CXC receptor-4 mRNA silencing abrogates CXCL12-induced migration of colorectal cancer cells
Control experiment | Type | Example |
|---|---|---|
Positive control siRNA | siRNA that is known to provide high knockdown of its target gene | Hs_MAPK1 siRNA (Qiagen) OrderNo. 1022564 |
Negative control siRNA | a nonsilencing siRNA with no homology to any known mammalian gene | AllStars Negative control siRNA (Qiagen) OrderNo. 1027280 |
Transfection control siRNA | siRNA that is used to measure the transfection efficiency, e.g. by siRNA | AllStars Hs Cell Death Control siRNA (Qiagen) OrderNo. 1027298 |
Mock transfection control | control experiment where cells go through the transfection process without addition of siRNA | Â |
Untransfected cells control | control experiment where gene expression analysis is carried out on cells that have not gone through the transfection process | Â |