Figure 1

Th17 cells and IL-17 are increased in CD patient tissue. a) Lamina propria mononuclear cells from control colon tissues or CD colons were cultured for two days with IL-1 and IL-23. Supernatants were subjected to ELISA for analysis of IL-17 protein. b) Serum from healthy or CD patients was subjected to ELISA for IL-17 quantification. n = 16. *P < 0.0001. c) Healthy blood or control colon tissue or CD colon tissue cells were stained with antibodies to CD3, CD4, IL-17, and FoxP3 and antigen expression was analyzed via FACS. Results are expressed as the percent of IL-17⁺ or Foxp3⁺ T cells in the CD4⁺ T cells. The gates were on CD4⁺CD3⁺ T cells for both Th17 and Treg cells. n = 16.