Figure 6
From: ATP synthase ecto-α-subunit: a novel therapeutic target for breast cancer
Anti-ATP synthase α-subunit antibody regulated apoptosis pathway of MDA-MB-231 cells. Gene's expression of MDA-MB-231cells was measured using SABioscience Human Apoptosis PCR Array PAHS-3012. Three samples were analyzed: Control group, MDA-MB-231 cells treated with IgG for 24 hours; Test group 1, MDA-MB-231 cells treated with MAb9E10 100 mg/ml for 24 hours; Test group 2, MDA-MB-231 cells treated with MAb9E10 100 mg/ml for 48 hours. Calculate the ΔCt for each pathway-focused gene in each treatment group. ΔCt (group) = average Ct - average of HK genes' Ct for group array. Calculate the ΔΔCt for each gene across two PCR Arrays (or groups). ΔΔCt = ΔCt (Test group) - ΔCt (Control group). Calculate the fold-change for each gene from Test group to control group as 2-ΔΔCt. A, Gene expression fold difference between Test group 1 and Control group. B, Gene expression fold difference between Test group 2 and Control group.