Figure 3

IAA levels peak at age 8-10 weeks for NOD that become diabetic by 20 weeks. IAA levels were followed in NOD mice from 4 weeks up to 36 weeks. A) IAA values from mice that developed diabetes by 20 weeks are plotted, with each line depicting the data from a single mouse. The IAA levels from mice that had an IAA value below the positive predictive threshold at 8.5 weeks (described in Table 1) are represented by dotted lines. The 95% confidence interval of IAA values from mice not diabetic by 20 weeks (shaded region, n = 22-29) is plotted for comparison. B) IAA values were normalized to the highest IAA value for each mouse (n = 18-25). The average (avg) IAA values at the indicated ages are graphed. Data after the age of 18 weeks are calculated from an avg of less than 10 mice due to euthanasia of diabetic mice. C) Percent of diabetes-free mice from 4 weeks up to 20 weeks are plotted (n = 54). The number of mice that became diabetic at each time point is indicated by bars. IAA value is calculated as the average signal from triplicate wells divided by the background signal from triplicate wells with negative control sera.