Figure 2

Murine IAA ECL assay has a broad dynamic range and negligible background from non-diabetic strains. A) The chemistry behind ECL technology, demonstrating the regenerating electrochemiluminescent reaction. A magnet below the electrode attracts the magnetic beads, bringing the Ru-tagged immune complex near the electrode. The Ru is then oxidized by the current and reacts with the free radical form of tripropylamine (TPA) to produce a photon. Superscripted asterisk indicates the higher energy state of the chemical and superscripted dot indicates a radical species. Bipyridine = bpy. B) The murine IAA ECL was tested using an anti-insulin mouse (Ms) monoclonal antibody or an isotype control antibody at the same concentrations. C) The murine IAA ECL assay was also performed on serum from 8-week-old NOD mice (n = 10) and non-diabetic control strains (C57BL/6 and BALB/c, n = 10 each). ECL S/N is the average signal from triplicate wells divided by the "noise" (background signal) from triplicate wells with beads alone.