Figure 7
Granzyme B production from STEAP 281-296 - or EZH2 95-109 -reactive CD4 T-cell clones. STEAP281-296-reactive, DR15-restricted CD4 T-cell clone SH31 (A, left) and DR53-restricted CD4 T-cell clone Sa12 (A, right) were able to secrete granzyme B reacting with STEAP+/HLA-DR matched LC cells. EZH295-109-reactive, DR15-restricted CD4 T-cell clone SK32 (B, left) and Sa361 (B, middle), and DR53-restricted CD4 T-cell clone Sa362 (B, right) were able to secrete granzyme B reacting with EZH2+/HLA-DR matched LC cells. The HLA-DR-negative Jurkat was also used as negative controls. The antigen specificity of these responses was demonstrated by blocking tumor recognition with L243. Columns without bars had SD < 10% the values of the mean. Results are representative of two separate experiments.