A. Percentage of CD8+ T cells# | |||
---|---|---|---|
CD8+ T cells (day 0: 20.22 ± 1.89) | |||
day 7 | day 14 | day 21 | |
NC | 25.48 ± 1.93 | 33.80 ± 20.15 | 41.92 ± 23.91 |
HSP70 | 25.20 ± 2.50 | 36.52 ± 12.65 | 47.70 ± 28.96 |
CMVpp65495-503 peptide | 27.07 ± 1.22 | 61.43 ± 11.61 | 63.06 ± 25.79 |
HSP70/CMV-PC | 27.27 ± 0.95 | 79.83 ± 11.36** | 83.17 ± 5.30* |
#cells were gated on viable lymphocytes | |||
B. Percentage of CD25 + , CD69 + , and CD137 + T cells â–¡ | |||
CD25+ T cells (day 0: 1.29 ± 0.21) | |||
day 7 | day 14 | day 21 | |
NC | 0.17 ± 0.29 | 0.23 ± 0.10 | 0.08 ± 0.04 |
HSP70 | 0.13 ± 0.23 | 0.28 ± 0.15 | 0.18 ± 0.08 |
CMVpp65495-503 peptide | 0.77 ± 0.29 | 36.18 ± 13.12 | 5.40 ± 5.29 |
HSP70/CMV-PC | 1.74 ± 0.81 | 38.25 ± 8.16 | 18.10 ± 0.57* |
CD69+ T cells (day 0: 13.62 ± 4.70) | |||
NC | 0.10 ± 0.10 | 0.23 ± 0.10 | 0.18 ± 0.18 |
HSP70 | 0.10 ± 0.10 | 0.30 ± 0.14 | 0.24 ± 0.27 |
CMVpp65495-503 peptide | 0.73 ± 0.22 | 2.68 ± 1.18 | 3.68 ± 4.18 |
HSP70/CMV-PC | 1.33 ± 0.59 | 2.18 ± 0.35 | 5.82 ± 7.71 |
CD137+ T cells (day 0: 2.39 ± 0.01) | |||
NC | 0.10 ± 0.10 | 0.10 ± 0.08 | 0.04 ± 0.05 |
HSP70 | 0.07 ± 0.06 | 0.15 ± 0.06 | 0.14 ± 0.09 |
CMVpp65495-503 peptide | 0.70 ± 0.17 | 12.77 ± 4.24 | 6.60 ± 1.39 |
HSP70/CMV-PC | 1.50 ± 0.52* | 25.10 ± 4.67* | 14.15 ± 5.87* |
â–¡cells were gated on CD8+ T cells | |||
C. Percentage of CD25 + , CD69 + , and CD137 + T cells â—‹ | |||
CD25+ T cells (day 0: 5.98 ± 0.33) | |||
day 7 | day 14 | day 21 | |
NC | 51.03 ± 44.41 | 49.46 ± 43.74 | 51.03 ± 56.44 |
HSP70 | 49.57 ± 43.27 | 48.62 ± 45.50 | 50.50 ± 55.42 |
CMVpp65495-503 peptide | 87.37 ± 2.90 | 72.90 ± 25.77 | 73.03 ± 45.76 |
HSP70/CMV-PC | 89.50 ± 5.40 | 78.55 ± 24.43 | 72.40 ± 47.37 |
CD69+ T cells (day 0: 16.49 ± 10.89) | |||
NC | 5.45 ± 4.74 | 10.73 ± 4.10 | 2.63 ± 4.56 |
HSP70 | 5.85 ± 3.89 | 12.08 ± 6.67 | 6.17 ± 8.45 |
CMVpp65495-503 peptide | 84.30 ± 0.52 | 2.93 ± 1.31 | 0.50 ± 0.44 |
HSP70/CMV-PC | 84.93 ± 12.00 | 3.07 ± 1.24 | 0.63 ± 0.58 |
CD137+ T cells (day 0: 11.86 ± 1.01) | |||
NC | 2.60 ± 2.40 | 4.23 ± 3.10 | 3.30 ± 1.87 |
HSP70 | 4.00 ± 3.82 | 8.45 ± 4.63 | 4.83 ± 3.61 |
CMVpp65495-503 peptide | 88.05 ± 3.04 | 15.45 ± 7.19 | 10.73 ± 8.39 |
HSP70/CMV-PC | 92.45 ± 5.87 | 17.05 ± 14.79 | 11.45 ± 9.73 |