Heat shock protein 70/peptide complexes: potent mediators for the generation of antiviral T cells particularly with regard to low precursor frequencies

Table 1 Classification of the three responder groups (weak, medium, and strong) after stimulation with CMVpp65_495-503 peptide.

	day 0		day 7
donor	percentage of pentamer⁺CD8⁺ T cells	absolute number of pentamer⁺CD8⁺ T cells [x10⁶]	percentage of pentamer⁺CD8⁺ T cells	absolute number of pentamer⁺CD8⁺ T cells [x10⁶]	responder classification
1	0.40	0.04	0.30	0.02	weak
2	0.39	0.04	0.19	0.02	weak
3	0.39	0.04	0.34	0.06	weak
4	0.37	0.04	0.50	0.07	weak
5	0.40	0.04	0.14	0.02	weak
6	0.30	0.03	4.50	0.56	medium
7	0.53	0.05	5.19	0.36	medium
8	2.17	0.22	2.75	0.17	medium
9	0.79	0.08	2.89	0.38	medium
10	0.30	0.03	1.11	0.19	medium
11	0.65	0.07	37.76	4.34	strong
12	5.64	0.56	13.54	1.15	strong
13	0.56	0.06	21.00	1.26	strong
14	1.40	0.14	16.67	2.17	strong
15	0.70	0.07	16.80	1.60	strong
16	1.70	0.17	19.50	1.37	strong

The data sets from 16 healthy HLA-A*02:01/CMV-seropositive donors were divided into 3 responder groups: weak (n = 5), medium (n = 5), and strong (n = 6), respectively, according to the obtained frequency of generated A2/CMV-pentamer-positive CD8⁺ T cells after 7 days of stimulation with the CMVpp65_495-503 peptide (weak responders < 1% A2/CMV-pentamer-positive CD8⁺ T cells, medium responders 1-10% A2/CMV-pentamer-positive CD8⁺ T cells, strong responders > 10% A2/CMV-pentamer-positive CD8⁺ T cells).

ISSN: 1479-5876