Expression of MR, 11β-HSD2 or CYP11B2 in cultured HMC, PTEC and kidney biopsies. (A) HMC expressed MR, 11β-HSD2 and CYP11B2 mRNAs and PTEC expressed only MR mRNA. Result was expressed as the mean fold change ± SD (related to the mean CT value from positive control using purified kidney total mRNA) from five individual experiments. (B) Demonstration of MR expression (arrows) in cultured PTEC by immunofluorescence staining (magnification × 200). (C). Detection of MR, 11β-HSD2 and CYP11B2 in kidney biopsies from IgAN patients and controls. Immunoreactive MR was located in both the glomeruli and tubules. Signal from immunoreactive 11β-HSD2 and CYP11B2 was located in glomeruli but not tubules (magnification × 200). Compared to staining results in control biopsies, increased (D) glomerular CYP11B2 staining and (E) tubular MR staining in IgAN patients were semi-quantified using a five-point scale.