Comparison of MIG and IP-10 induction in whole blood vs. PBMCs. Whole blood (100 μl) clear bars or PBMCs (2 × 105) filled bars were incubated with peptides spanning the EBV antigen BZLF1, human actin (ACTs) or recombinant IFNγ (10,000pg/ml) for 18-20 hours before lysis and RNA harvesting. The induction of MIG (Panel A) and IP-10 (Panel B) gene expression are shown normalized to the negative control (ACTs). Columns show the mean RQ value and 95% confidence intervals calculated from triplicate wells. Results are representative of five individual experiments.