Figure 3
Policlonal BV repertoire expansions are found in the pancreata following B cell reconstitution in NOD.Igμnull mice. A) Spectratype analysis of BV-BC (Vbeta-Cbeta) expansions for pancreas-infiltrating T cells 10 weeks post-B cell reconstitution demonstrate a polyclonal profile of induced clonotypes, with BV2, BV10, BV12 and BV14 being present on over 60% of the animals, followed next in appearance by BV8S3 and BV11, present in 50% of the mice. B) As disease progresses, a higher diversity of clonotypes is observed, particularly for the appearance of BV16, BV17, BV18, BV19 and BV20 in 13-16 weeks post-reconstitution and later C) at pre-diabetic stages (19-31 weeks post-reconstitution).