Table 2 Viability after electroporation, cryopreservation and thawing
From: Three-day dendritic cells for vaccine development: Antigen uptake, processing and presentation
3d mDC | 7d mDC | ||||||
|---|---|---|---|---|---|---|---|
w/o EP | 300 V 300 μF | 250 V 150 μF | w/o EP | 300 V 300 μF | 250 V 150 μF | ||
Donor 3 | Before freezing | ||||||
Cell counts (× 106) | 1.4 | 0.5 | 0.8 | 0.7 | 0.5 | 0.4 | |
Viability* | 94% | 90% | 91% | 75% | 52% | 74% | |
After thawing | |||||||
Cell counts (× 106) | 0.5 | 0.2 | 0.2 | 0.2 | 0.1 | 0.2 | |
Viability* | 86% | 79% | 78% | 51% | 26% | 55% | |
Donor 4 | Before freezing | ||||||
Cell counts (× 106) | 1.1 | 1.4 | 1.3 | 0.7 | 0.7 | 0.6 | |
Viability* | 89% | 85% | 88% | 59% | 58% | 62% | |
After thawing | |||||||
Cell counts (× 106) | 1.0 | 0.9 | 0.8 | 0.6 | 0.4 | 0.5 | |
Viability* | 90% | 90% | 89% | 86% | 79% | 84% | |