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Table 2 Viability after electroporation, cryopreservation and thawing

From: Three-day dendritic cells for vaccine development: Antigen uptake, processing and presentation

   3d mDC 7d mDC
   w/o EP 300 V 300 μF 250 V 150 μF w/o EP 300 V 300 μF 250 V 150 μF
Donor 3 Before freezing       
  Cell counts (× 106) 1.4 0.5 0.8 0.7 0.5 0.4
  Viability* 94% 90% 91% 75% 52% 74%
  After thawing       
  Cell counts (× 106) 0.5 0.2 0.2 0.2 0.1 0.2
  Viability* 86% 79% 78% 51% 26% 55%
Donor 4 Before freezing       
  Cell counts (× 106) 1.1 1.4 1.3 0.7 0.7 0.6
  Viability* 89% 85% 88% 59% 58% 62%
  After thawing       
  Cell counts (× 106) 1.0 0.9 0.8 0.6 0.4 0.5
  Viability* 90% 90% 89% 86% 79% 84%
  1. * Viability: PI stain