Figure 1

Transfection of AS ODN for PAR-2 inhibits the expressiongn of PAR-2 in H. pylori -infected AGS cells. AGS cells were transfected with PAR-2 AS ODN or S ODN for 24 hours and cultured in the absence or the presence of H. pylori at a bacterium/cells ratio of 300:1 for 2 hours. The levels of PAR-2 were determined by Western blot analysis using specific antibody for PAR-2. Actin served as a loading control. Wild, the cells without transfection; Transfected, the cells transfected with S ODN (S) or AS ODN (AS). None, the cells without transfection and cultured in the absence of H. pylori; H. pylori control, the cells without transfection and cultured in the presence of H. pylori.