Figure 1

Expression of CDC25B in different ESCC cell lines and optimization of antigen concentration for ELISA assays. A. Expression of CDC25B in ESCC cell lines was examined by Western blot analysis with an anti-CDC25B antibody (N: normal esophageal tissue). B. Effect of different amounts of Eca-109 total protein on absorbance at 450 nm in CDC25B-Abs reverse capture ELISA. Twenty samples from patients with ESCC and twenty samples from healthy controls were tested in reverse capture ELISAs using different amounts of Eca-109 total protein as antigen. The results shown are the mean values of three experiments.