Skip to main content

Table 1 Description of viruses used in the study

From: Adenoviruses with an αvβ integrin targeting moiety in the fiber shaft or the HI-loop increase tumor specificity without compromising antitumor efficacy in magnetic resonance imaging of colorectal cancer metastases

Virus Capsid modification References
Replication deficient viruses a AdTL Wild type 5 capsid  
  DATL -Y477A substitution in DE loop of fiber knob for CAR ablation  
   -Penton base's RGD domain mutated to RGE for αvβ integrin ablation [41]
   -6xhistidine carboxy-terminal tag for the propagation in 293.HissFv.rec cells  
  AdTLG -Fiber shaft's KKTK domain mutated to GATK for HSPG ablation [27]
  AdTLGR -RGD insertion in HI loop of fiber knob for αvβ integrin targeting  
   -Fiber shaft's KKTK domain mutated to GATK for HSPG ablation [27]
  AdTLYG -Y477A substitution in DE loop of fiber knob for CAR ablation  
   -Fiber shaft's KKTK domain mutated to GATK for HSPG ablation [21, 27]
  AdTLYGR -Y477A substitution in DE loop of fiber knob for CAR ablation  
   -RGD insertion in HI loop of fiber knob for αvβ integrin targeting  
   -Fiber shaft's KKTK domain mutated to GATK for HSPG ablation [21, 27]
  AdTLY -Y477A substitution in DE loop of fiber knob for CAR ablation [21]
  Ad5luc1RGD -RGD insertion in HI loop of fiber knob for αvβ integrin targeting [42]
  AdTLRGDK -Fiber shaft's KKTK domain mutated to RGDK for αvβ integrin targeting [30]
   -HSPG ablation via mutated KKTK  
Replicating viruses WT -Replicating wild type 5 virus  
  WT-RGD -RGD insertion in HI loop of fiber knob for αvβ integrin targeting [43]
  WT-RGDK -Fiber shaft's KKTK domain mutated to RGDK for αvβ integrin targeting [30]
   -HSPG ablation via mutated KKTK  
  1. aAll replication deficient viruses are deleted for E1A and have both luciferase (Luc) and green fluorescent protein (GFP) as marker genes.
  2. CAR, coxsackie virus and adenovirus receptor; HSPG, heparan sulphate proteoglycan; VP, viral particles; pfu, plaque forming unit.