Adenoviruses with an αvβ integrin targeting moiety in the fiber shaft or the HI-loop increase tumor specificity without compromising antitumor efficacy in magnetic resonance imaging of colorectal cancer metastases

Table 1 Description of viruses used in the study

Virus		Capsid modification	References
Replication deficient viruses ^a	AdTL	Wild type 5 capsid
	DATL	-Y477A substitution in DE loop of fiber knob for CAR ablation
		-Penton base's RGD domain mutated to RGE for α_vβ integrin ablation	[41]
		-6xhistidine carboxy-terminal tag for the propagation in 293.HissFv.rec cells
	AdTLG	-Fiber shaft's KKTK domain mutated to GATK for HSPG ablation	[27]
	AdTLGR	-RGD insertion in HI loop of fiber knob for α_vβ integrin targeting
		-Fiber shaft's KKTK domain mutated to GATK for HSPG ablation	[27]
	AdTLYG	-Y477A substitution in DE loop of fiber knob for CAR ablation
		-Fiber shaft's KKTK domain mutated to GATK for HSPG ablation	[21, 27]
	AdTLYGR	-Y477A substitution in DE loop of fiber knob for CAR ablation
		-RGD insertion in HI loop of fiber knob for α_vβ integrin targeting
		-Fiber shaft's KKTK domain mutated to GATK for HSPG ablation	[21, 27]
	AdTLY	-Y477A substitution in DE loop of fiber knob for CAR ablation	[21]
	Ad5luc1RGD	-RGD insertion in HI loop of fiber knob for α_vβ integrin targeting	[42]
	AdTLRGDK	-Fiber shaft's KKTK domain mutated to RGDK for αvβ integrin targeting	[30]
		-HSPG ablation via mutated KKTK
Replicating viruses	WT	-Replicating wild type 5 virus
	WT-RGD	-RGD insertion in HI loop of fiber knob for α_vβ integrin targeting	[43]
	WT-RGDK	-Fiber shaft's KKTK domain mutated to RGDK for α_vβ integrin targeting	[30]
		-HSPG ablation via mutated KKTK

^aAll replication deficient viruses are deleted for E1A and have both luciferase (Luc) and green fluorescent protein (GFP) as marker genes.
CAR, coxsackie virus and adenovirus receptor; HSPG, heparan sulphate proteoglycan; VP, viral particles; pfu, plaque forming unit.

ISSN: 1479-5876