Figure 2

IMP321 increases the numbers of monocytes, NK and activated CD8 T cells in blood (panel A). Fresh blood samples were collected pre-dosing, at D1, D85 and D170 and directly stained with fluorochrome-conjugated antibodies in tubes containing a precise number of fluorescent control beads. The results show the mean ± sd of the absolute numbers of CD45⁺CD14⁺ (monocytes), CD3^-CD56⁺ (NK cells) and CD38⁺HLA-DR⁺CD8⁺ (activated CD8⁺ cells) cells. The paired non-parametric Wilcoxon signed rank test was used to compare increases observed between D85 or D170 and D1. When significant (< 0.05), p values are indicated. IMP321 increases the percentages of dendritic cells and cytotoxic CD45RA⁺ Effector-Memory CD8⁺ T cells (EMRA) in PBMCs (panel B). PBMCs cells collected pre-dosing, at D1, D85 and D170 were isolated and stained with fluorochrome-conjugated antibodies and analyzed by flow cytometry. The results show the mean ± sd of the percentages of plasmacytoid dendritic cells (pDC, CD45⁺CD14^-CD16^-HLA-DR⁺CD123⁺CD11c^-) and myeloid dendritic cells (mDC, CD45⁺CD14^-CD16^-HLA-DR⁺CD123^-CD11c⁺) in CD45⁺ cells, as well as the percentages of CD45RA⁺CD45RO^-CD62L^- in the CD8⁺ T cell population (CD45RA⁺EM CD8 T cells or EMRA). The significant Wilcoxon p values are indicated.