Table 1 Incidence of V600 mutations in melanoma patients.
Total V600 mutants | V600E (%) | V600K (%) | V600 D or V600R (%) | Reference | Assay |
|---|---|---|---|---|---|
34 | 25 (73.5) | 4 (11.8) | 5 (14.7) | Spittle et al. [7] | Pyrosequencing, validated by Sanger dideoxy sequencing |
10 | 9 (90.0) | 1 (10.0) | 0 (0.0) | Hay et al. [8] | Melting point analysis, validated by Sanger dideoxy sequencing |
44 | 34 (77.3) | 9 (20.5) | 1 (2.3) | Willmore-Payne et al. [9] | Amplicon melting analysis, validated by Sanger dideoxy sequencing |
42 | 29 (69) | 12 (28.6) | 1 (2.3) | Halaban et al. [5] and Halaban, unpublished | Sanger dideoxy sequencing |
50 | 47 (94.0) | 3 (6.0) | 0 (0.0) | Ugurel et al. [10] | Fluorescent capillary SSCP technique |
178 | 143 (80.3) | 29 (16.3) | 6 (3.4) | Total Mutations | Â |