Figure 6

Cellular expressions of CXCR4 and stromal derived factor (SDF)-1 in brain infarction area. Immunohistofluorescence (IHF) staining (400×) showing substantially lower number of CXCR4-positive cells (red arrows) in group 1 (A) than in groups 2 (B) and 3 (C), and remarkably lower in group 2 than in group 3; (D) indicated quantification results of each group (n = 10). ‡ vs. † vs. *, p < 0.001. IHF staining (400×) also demonstrating significantly lower number of SDF-1 positive cells (yellow arrows) in group 1 (E) than in groups 2 (F) and 3 (G), and notably lower in group 2 than in group 3. (H) indicated quantification results of each group (n = 10). ‡ vs. † vs. *, p < 0.001. Western blotting showing markedly lower CXCR4 (I) and SDF-1 (J) protein expressions in group 1 than in groups 2 and 3, and notably lower in group 2 than in group 3. n = 10 per group. Scale bars in right lower corner represent 50 μm. * vs. † vs. ‡, p < 0.01.