Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood

Table 4 Inhibition by Ab-01 of ex vivo rhIL21-dependent IL2RA expression

Animal	rHuIL21 alone	rHuIL21 + Control IgG₁TM	rHuIL21 + Ab-01
1	4.7	3.5	1.2
2	3.8	3.8	0.9
3	4.1	5.1	1.4
4	2.8	2.8	0.9
5	3.3	3.2	1.3
6	2.8	2.9	1
7	3.6	3.7	1.1
8	7.2	8.8	1.1

Each value represents the fold change over the no-treatment group in expression levels of IL2RA in monkey blood samples following 4 hour ex vivo treatment as indicated.

ISSN: 1479-5876

Animal	rHuIL21 alone	rHuIL21 + Control IgG₁TM	rHuIL21 + Ab-01
1	4.7	3.5	1.2
2	3.8	3.8	0.9
3	4.1	5.1	1.4
4	2.8	2.8	0.9
5	3.3	3.2	1.3
6	2.8	2.9	1
7	3.6	3.7	1.1
8	7.2	8.8	1.1

Animal	rHuIL21 alone	rHuIL21 + Control IgG₁TM	rHuIL21 + Ab-01
1	4.7	3.5	1.2
2	3.8	3.8	0.9
3	4.1	5.1	1.4
4	2.8	2.8	0.9
5	3.3	3.2	1.3
6	2.8	2.9	1
7	3.6	3.7	1.1
8	7.2	8.8	1.1

Animal	rHuIL21 alone	rHuIL21 + Control IgG₁TM	rHuIL21 + Ab-01
1	4.7	3.5	1.2
2	3.8	3.8	0.9
3	4.1	5.1	1.4
4	2.8	2.8	0.9
5	3.3	3.2	1.3
6	2.8	2.9	1
7	3.6	3.7	1.1
8	7.2	8.8	1.1