Figure 2

HSF1 overexpression increases the oncolytic effect of Adel55 in Bcap37 cells. (A) Western blot shows the levels of HSF1, Hsp90, Hsp70, Hsp27 in Bcap37 and the stable cell lines Bcap37/LXSN, Bcap37/cHSF1, Bcap37/HSF1i. Actin expression was used as a loading control. (B) The cytotoxicity of Adel55 on the indicated cell lines. Cells were infected with Adel55 at MOI of 0.1, 1, or 10. 7 days later, cell viability assay was performed. Meanwhile, another set of cells were infected with Adel55 at MOI of 10. 1, 3, 5 or 7 days later, cell viability assay was performed. The data represent the mean ± SD of four repeats.