Figure 1

The oncolytic effect of Adel55 correlates with the intracellular HSF1 activity. (A) Schematic structure of Ad (E1A-), Adel55 and Adel55-gene. In Ad (E1A-), the E1A gene was deleted. In Adel55, the E1B55kDa gene was replaced by SV40 polyA. In Adel55-gene, the expression box of the foreign gene (EGFP, cHSF1, or HSF1i) was inserted into the Bgl II site after SV40 polyA. Wild type Ad5 structure was also depict. (B) The cytotoxicity of Ad (E1A-) and Adel55. Cells were infected with Ad (E1A-) or Adel55 at MOI of 10. Cell viability assay were performed by MTT assay at different time points. The cell survival rate of mock-infected cells was defined as 100%. The data represent the mean ± SD of three determinations. (C) Assessment of HSE activity in tumor or normal cell lines. Cells were transfected with luciferase reporter plasmid, pHSE-luc. Luciferase assays were performed 48 h later. The values (mean ± SD for four assays) are represented as relative light units/mg of protein. The cells transfected by pGL3-Basic without promoter were used as a negative control.