CD14 expression in the liver tissues of rats. CD14 staining in the liver tissues of rats from the sham and BDL groups. Staining of liver sections using a polyclonal antibody against CD14 shows negligible or no staining in any liver cells in the control (A). Positive staining in the Kupffer cells (arrow), the sinusoidal endothelial cells (arrowhead) and more strongly in hepatocytes around the portal zones at 3 h after sham-operation (B), and at 3 h, 1 d, 1w, 3w (C-F) after BDL. Tissues were counterstained with hematoxylin. Original magnification: × 200. The ratio of CD14 activated (CD14 positive cells were present in over 10% of the tissue area) of the sham (pale bar) and BDL (black bar) groups (G).