Total bilirubin and ALT levels in rats. Time course of total bilirubin (T-bilirubin; square) and alanine transaminase (ALT; circle) in rat plasma after bile duct ligation (BDL; closed symbols) or sham (open symbols) operation. Blood samples were collected at the time points indicated. T-bilirubin and ALT were assayed using a biochemistry auto-analyzer (Model 7450; Hitachi, Tokyo, Japan). Values are mean ± SD (n = 6 in each subgroup). *p < 0.05, **p < 0.005 (sham vs. BDL groups).