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Table 1 LC/ESI-MS/MS identification of immunoaffinity purified forms of gelsolin

From: Immunoreactivity of anti-gelsolin antibodies: implications for biomarker validation

Gel band M.W.* Theoretical minimum M.W. ** Peptide sequence Peptide position in pGSN
Band 1 86 kDa 65816.00 kDa EVQGFESATFLGYFK 121 - 135
    HVVPNEVVVQR 151 - 161
    PALPAGTEDTAKEDAANR 251 - 268
    QTQVSVLPEGGETPLFK 347 - 363
    DPDQTDGLGLSYLSSHIANVER 371 - 392
    AGALNSNDAFVLK 558 - 570
    TPSAAYLWVGTGASEAEK 571 - 588
    AQPVQVAEGSEPDGFWEALGGK 600 - 621
    DSQEEEKTEALTSAK 687 - 701
    RYIETDPANR 702 - 711
    RTPITVVK 714 - 721
Band 2 83 kDa 50126.19 kDa PALPAGTEDTAK 251 - 262
    QTQVSVLPEGGETPLFK 347 - 363
    DPDQTDGLGLSYLSSHIANVER.V 371 - 393
    AQPVQVAEGSEPDGFWEALGGK.A 600 - 621
    DSQEEEKTEALTSAK 687 - 701
    YIETDPANR 703 - 711
Band 3 64 kDa 64805.80 kDa R.EVQGFESATFLGYFK.S 120 - 136
    K.PALPAGTEDTAK.E 250 - 263
    K.QTQVSVLPEGGETPLFK.Q 346 - 364
    K.DSQEEEKTEALTSAK.R 686 - 702
    R.YIETDPANR.D 702 - 712
    -.YIETDPANR.- 703 - 711
Band 4 60 kDa 49194.23 kDa K.PALPAGTEDTAK.E 250 - 263
    R.DPDQTDGLGLSYLSSHIANVER.V 370 - 393
    K.AGALNSNDAFVLK.T 557 - 571
    R.AQPVQVAEGSEPDGFWEALGGK.A 599 - 622
    K.DSQEEEKTEALTSAK.R 686 - 702
Band 5 54 kDa 41077.02 kDa K.QTQVSVLPEGGETPLFK.Q 346 - 364
    R.DPDQTDGLGLSYLSSHIANVER.V 370 - 393
    K.VPVDPATYGQFYGGDSYIILYNYR.H 430 - 455
    K.AGALNSNDAFVLK.T 557 - 571
    -.TGAQELLR.- 589 - 596
    R.AQPVQVAEGSEPDGFWEALGGK.A 599 - 622
    K.DSQEEEKTEALTSAK.R 686 - 702
    -.RTPITVVK.- 714 - 721
Band 6 45 kDa 36044.42 kDa R.VPFDAATLHTSTAMAAQHGMDDDGTGQK.Q 392 - 421
    K.VPVDPATYGQFYGGDSYIILYNYR.H 430 - 455
    K.AGALNSNDAFVLK.T 557 - 571
    K.TPSAAYLWVGTGASEAEK.T 570 - 589
    R.AQPVQVAEGSEPDGFWEALGGK.A 599 - 622
    K.DSQEEEKTEALTSAK.R 686 - 702
    R.RYIETDPANR.D 701 - 712
    R.YIETDPANR.D 702 - 712
    R.RTPITVVK.Q 713 - 722
Band 7 27 kDa 30525.25 kDa VPVDPATYGQFYGGDSYIILYNYR 431 - 454
    AGALNSNDAFVLK 558 - 570
    RYIETDPANR 702 - 711
Band 8 19 kDa 29308.93 kDa VPVDPATYGQFYGGDSYIILYNYR 431 - 454
    DSQEEEKTEALTSAK 687 - 701
  1. Included is the band identification (corresponding to extracted bands in Figure 1, column A), molecular weight based on electrophoretic mobility, theoretical minimum molecular weight as calculated using ExPASy Compute pI/Mw tool, identified peptides, and peptide location in secreted pGSN. The peptide -DSQEEKTEALTSAK- was the most commonly identified peptide (in 7 of 8 bands). * - Molecular weight (M.W.) is approximate based on electrophoretic mobility in 1DE SDS-PAGE. ** - Theoretical Molecular weight was approximated using the ExPASy Compute pI/Mw tool and was calculated using the first peptide position through the last peptide position as determined using MS/MS.