Table 1 LC/ESI-MS/MS identification of immunoaffinity purified forms of gelsolin
From: Immunoreactivity of anti-gelsolin antibodies: implications for biomarker validation
Gel band | M.W.* | Theoretical minimum M.W. ** | Peptide sequence | Peptide position in pGSN |
|---|---|---|---|---|
Band 1 | 86 kDa | 65816.00 kDa | EVQGFESATFLGYFK | 121 - 135 |
HVVPNEVVVQR | 151 - 161 | |||
PALPAGTEDTAKEDAANR | 251 - 268 | |||
QTQVSVLPEGGETPLFK | 347 - 363 | |||
DPDQTDGLGLSYLSSHIANVER | 371 - 392 | |||
AGALNSNDAFVLK | 558 - 570 | |||
TPSAAYLWVGTGASEAEK | 571 - 588 | |||
AQPVQVAEGSEPDGFWEALGGK | 600 - 621 | |||
DSQEEEKTEALTSAK | 687 - 701 | |||
RYIETDPANR | 702 - 711 | |||
RTPITVVK | 714 - 721 | |||
Band 2 | 83 kDa | 50126.19 kDa | PALPAGTEDTAK | 251 - 262 |
QTQVSVLPEGGETPLFK | 347 - 363 | |||
DPDQTDGLGLSYLSSHIANVER.V | 371 - 393 | |||
AQPVQVAEGSEPDGFWEALGGK.A | 600 - 621 | |||
DSQEEEKTEALTSAK | 687 - 701 | |||
YIETDPANR | 703 - 711 | |||
Band 3 | 64 kDa | 64805.80 kDa | R.EVQGFESATFLGYFK.S | 120 - 136 |
K.PALPAGTEDTAK.E | 250 - 263 | |||
K.QTQVSVLPEGGETPLFK.Q | 346 - 364 | |||
K.DSQEEEKTEALTSAK.R | 686 - 702 | |||
R.YIETDPANR.D | 702 - 712 | |||
-.YIETDPANR.- | 703 - 711 | |||
Band 4 | 60 kDa | 49194.23 kDa | K.PALPAGTEDTAK.E | 250 - 263 |
R.DPDQTDGLGLSYLSSHIANVER.V | 370 - 393 | |||
K.AGALNSNDAFVLK.T | 557 - 571 | |||
R.AQPVQVAEGSEPDGFWEALGGK.A | 599 - 622 | |||
K.DSQEEEKTEALTSAK.R | 686 - 702 | |||
Band 5 | 54 kDa | 41077.02 kDa | K.QTQVSVLPEGGETPLFK.Q | 346 - 364 |
R.DPDQTDGLGLSYLSSHIANVER.V | 370 - 393 | |||
K.VPVDPATYGQFYGGDSYIILYNYR.H | 430 - 455 | |||
K.AGALNSNDAFVLK.T | 557 - 571 | |||
-.TGAQELLR.- | 589 - 596 | |||
R.AQPVQVAEGSEPDGFWEALGGK.A | 599 - 622 | |||
K.DSQEEEKTEALTSAK.R | 686 - 702 | |||
-.RTPITVVK.- | 714 - 721 | |||
Band 6 | 45 kDa | 36044.42 kDa | R.VPFDAATLHTSTAMAAQHGMDDDGTGQK.Q | 392 - 421 |
K.VPVDPATYGQFYGGDSYIILYNYR.H | 430 - 455 | |||
K.AGALNSNDAFVLK.T | 557 - 571 | |||
K.TPSAAYLWVGTGASEAEK.T | 570 - 589 | |||
R.AQPVQVAEGSEPDGFWEALGGK.A | 599 - 622 | |||
K.DSQEEEKTEALTSAK.R | 686 - 702 | |||
R.RYIETDPANR.D | 701 - 712 | |||
R.YIETDPANR.D | 702 - 712 | |||
R.RTPITVVK.Q | 713 - 722 | |||
Band 7 | 27 kDa | 30525.25 kDa | VPVDPATYGQFYGGDSYIILYNYR | 431 - 454 |
AGALNSNDAFVLK | 558 - 570 | |||
RYIETDPANR | 702 - 711 | |||
Band 8 | 19 kDa | 29308.93 kDa | VPVDPATYGQFYGGDSYIILYNYR | 431 - 454 |
DSQEEEKTEALTSAK | 687 - 701 |