Western blot titration of hGSN. Titration of hGSN using Western-blot assay shows limitations of this assay in quantitation of this protein. At higher concentrations other forms than full-length molecule are detected, however, 86 kDa band representing full-length molecule is oversaturated. Conversely, at low concentration, full-length molecule can be quantitated, but the presence of all other forms is missed. Goat anti-hGSN antibody was used in this experiment.