Figure 4

Abolishment of ADAM 10 expression in SACC-LM cells. (a) ADAM 10 mRNA levels were determined by qRT-PCR. Relative fold induction for the ADAM 10 mRNA (mean ± SD) in mock- and ADAM 10 siRNA-transfected cells is presented relative to the expression in parental SACC-LM cells (*p < 0.001 compared with SACC-LM). (b) Western blot analysis for ADAM 10 protein expression in the indicated cell lines. GAPDH was used as a loading control. SACC-LM (high metastatic potential control); SACC-Mock (mock transfection control); SACC-ADAM10-RNAi (1), (2), and (3) represent the three different clones, respectively.