Figure 8

Cytolytic activity of CIK cells generated with ^hiTG from patients with advanced solid cancer. Panel A: CIK cells were differentiated with ^hiTG from 4 patients with advanced solid cancer and were used to assess cytolytic activity against NK-sensitive tumor-targets. K562 CML cells were pre-loaded with CAM, a fluorescent probe, followed by their co-culture with CIK cells for 4 hours at the indicated E:T ratio. Contour plots depict the raw percentage of 7-AAD⁺CAM^int target cells that have been lysed at the end of the 4-hour co-culture. Quadrant markers were set according to the proper isotypic control (not shown), i.e., K562 cells that were neither loaded with CAM nor labeled with 7-AAD. Panel B: Cumulative cytotoxicity of CIK cells differentiated from the 4 patients with advanced solid cancer. Bars depict median values with interquartile range. The percentage of 7-AAD⁺ cells in cultures with K562 target cells alone (background cell death) is shown as uncolored column.