Phenotypic features of TG-expanded CIK cells, NK cells and CD8+ T cells. The frequency of CD3+CD8+ T cells, NK cells (CD3-CD16+CD56+) and CIK cells (CD3+CD16+CD56+) was measured by flow cytometry weekly after the provision of different concentrations of either TG or αCD3 mAb to the cultures. One experiment out of 10 with similar results is shown. Quadrant markers were set according to the proper isotypic control (not shown). The percentage of cells staining positively for a given antigen is indicated.