Figure 3

Comparison of T cell phenotype 14 and 21 days after primary stimulation of MNCs with CD3/CD28 beads and soluble anti-CD3. CD4 T cells (A) showed a similar pattern of changes in response to either stimulus, but CD8 cells (B) demonstrated significant differences in phenotype. At comparable levels of expansion, anti-CD3 treated CD8 cells retained higher levels of CD45RA, CD27, and CCR7 expression than anti-CD3/CD28 bead treated cells and bead-treated CD8 cells expressed higher levels of CD28.