Kaplan-Meier analysis of overall survival (OS) and disease-free survival (DFS). The median OS and DFS for 37 patients overall and 29 patients without distant metastasis were 19.0 and 10.0 months, respectively (A, E). GC patients with negative MMP9 expression (-) displayed better OS (B, upper curve) compared to those with positive MMP9 expression (B, lower curve) (P = 0.036, Log-rank test). Type IV collagen is a protective factor for GC patients (C, P = 0.044, Log-rank test). High MVD may predict poor OS (D, P = 0.001, Log-rank test). Low density of infiltrating Macrophages showed a tendency towards favorable DFS. Patients in low density of infiltrating macrophages group expression displayed improve DFS (F, upper curve) compared to patients with high density group expression (F, lower curve) (P = 0.013, Log-rank test).