Figure 6

Silencing and infection with Adeno5 of a MxA positive cell line: PaCa44. A) Activation of ISRE promoter (gray bars) and IFNbeta promoter (black bars) in MxA+ cell lines. The Y axes express the production of the reporter gene normalized by the same cell line carrying a plasmide with non-targeting control (NC). Please add n of experiments and error bars The data were normalized using a pMet luc plasmid control. B) ISG15 expression by Western Blot after 24 hours of silencing for NFkB, IRF7, IRF3, VISA, untreated, non-targeting control (NC), respectively. C) Decreased level of ISRE regulated reporter gene expression in PaCa44 cell line after silencing with IRF3, IRF7, NFkB or non-targeting control (NC). The data were normalized using a pMet luc plasmid control. D) FACS analysis profile of GFP expression in PaCa44 cell line infected with Adeno5 CMV-GFP virus after silencing IRF3 and IRF7. Cells were infected by using 136 pfu/cell: solid black line, 68 pfu/cell: dashed black line, 27.2 pfu/cell: dotted black line of Adeno5-CMV-GFP vector and 136 pfu/cell Adeno5-CMV-Null vector: solid grey. Numbers represent the MFI.