Figure 3

UCHL1 promoter DNA methylation in RCC lesions, tumor adjacent kidney epithelium and RCC cell lines. A) Representative COBRA analysis of three RCC tumor lesions and one RCC cell line. Genomic DNA extracted from tumor lesions (2874TU, 2876TU and 2878) and the cell line MZ1940RC was treated with bisulfite and amplified by nested PCR as described in the Methods section. The resulting 265 bp amplicons were either digested with Taq I (+) or left untreated (-) and subsequently separated in 2% agarose gels in TAE buffer. A 100 base pair DNA ruler loaded in the first lane served as length standard. B) Distribution pattern for UCHL1 promoter DNA methylation in tumor adjacent kidney epithelium, autologous primary RCC lesions and RCC cell lines. Grey bars represent samples with unmethylated (U), striped bars with partially methylated (P) and black bars with fully methylated (M) CpG islets within the UCHL1 promoter core region as indicated.