Figure 4
Tumors in high does ascorbic acid treated groups exhibit poorly formed. Histochemical (A, × 100 and B, × 200) and immunohistochemical data (C to F) of liver tissues represent the clear tumor staining in group e. A-B: Cancer induction was identified by H & E staining in liver tissues treated with ascorbic acid. Small letters in each figure (a to g) represent the name of each group. C-F: Expression of angiogenesis related proteins (bFGF, VEGF and MMP2) were examined by immunohistochemistry. The name of each tested groups were shown in (C to A group), (D to D group), (E to E group) and (F to F group) in Figures. Angiogenesis related proteins of group D showed dark brown stains rather than other tested groups.