Figure 4

Dystrophin IF in culture cells. Anti-human dystrophin (N-terminal) FITC conjugated (green fluorescence) and nuclei dyed with Bisbenzimide H33342 (blue fluorescence). a) normal muscle cells, 200×; b) muscle cells of patient affected by DMD (dystrophin absent), 200×; c) Co-culture of stem cells CD34+ and muscle cells of patient affected by DMD, 200×. Microscope Zeiss Axiovert 200.