Figure 5

BPA induces modulation of ERK and AKT phosphorylation and increase of histone acetylation in NB4 cells. (A) Western blot analysis showing ERK and AKT phosphorylation in NB4 cells treated with 60 μM BPA at the times indicated times; (B) Western blot analysis of the acetylation levels of Histone H3 in NB4 cells treated for 2, 4 and 6 days with 60 μM BPA. ERKs expression levels account for equal loading); (C) Western blot analysis of the phosphorylation levels of Rb and p53 expression in NB4 cells treated for 2, 4 and 6 days with 60 μM BPA. ERKs expression levels account for equal loading); (D) Western blot analysis of the expression levels of ER alpha in NB4 cells treated for 2, 4 and 6 days with 60 μM BPA. As positive control for the ER alpha detection (indicated as +) 25 μg of MCF7 protein extracts have been used. ERKs expression levels account for equal loading.