Figure 1

PAR1 expression in breast cancer patients. Western blots showing PAR and EGFR expression profiles of tumor biopsy samples from patients with infiltrating ductal carcinoma (Figure 1a and 1b). The blots are representative of three replicate tests. (c) A representative example of immunofluorecent staining of PAR1; Red line: background fluorescence (secondary antibody alone); green line: fluorescent shift attributable to PAR1 expression. Traces shown are representative of one of three independent measurements. (d) A tissue sample exhibiting PAR1 (visualized using ×10 and ×40, objective lens) and HER2 strong membrane immunostaining; also shown: H&E and a negative control sections.