Figure 4

Analysis of differentially expressed MPD neutrophil genes by quantitative real time PCR (RT-PCR). The expression of five genes NFKBIA, NFKBIE, TNFAIP3, MCL1 and CBS in MPD neutrophils was analyzed by qRT-PCR. The expression of NFKBIA, NFKBIE, and TNFAIP3 were down-regulated in MPD and G-CSF-mobilized neutrophils. The expression of CBS was significantly increased in MPD neutrophils. The expression of MCL1 was also increased in MPD neutrophils but the difference was not significant. The results of analysis by qRT-PCR and gene expression profiling were similar.